If the interesterification reaction of rapeseed oil with methyl acetate at reactant to oil molar ratio of 18:1 in presence of potassium tert-butoxide in tert-butanol of molar ratio to oil 0.08 is conducted at a temperature of about 35 oC, reaction time for full conversion of oil is shorter than one hour, while at a temperature of 55 oC it is approximately 15 minutes. Reaction time at the desired temperature has a wide “optimal” range and cannot be an effective variable for the process optimisation. Experimental results at the temperature of 25 oC confirm the pseudo-first order of the reaction, which lowered towards the end of the reaction. The pseudo-first order rate constant was 0.63 min-1. Fuel characteristics of the interesterification reaction mixtures without purification improved with the rising of reaction temperature from 35 oC to 55 oC, however, they fail to meet the requirements of standard EN14214 for biodiesel. Methyl acetate to oil molar ratio 18:1 is too low for obtaining products with kinematic viscosity below 5.0 mm2/s.